【Chemical Name】 Agarose
【Synonym】
TOPVISION(TM) LE GQ AGAROSE;LOW EEO AGAROSE;LOW ELECTROENDOSMOSIS AGAROSE;LOW-MELTING-POINT, MULTI-PURPOSE AGAROSE;4% Agarose gel;AGAROSE 1;AGAROSE 10;AGAROSE 15
【CAS No】 39346-81-1
【Molecular Formula】C24H38O19
【Molecular Weight】 0
【Properties】 Melting point ≤90 °C (4% in water)(lit.) density 1.00 g/ml at 20 ° C flash point 38 ° C
【Specification】≥99
【Packing】1kg/bag
【Usage】 1. After modification, the gel temperature and melting point are lower, which is convenient to recover and purify DNA from low melting point agarose gel. Radiolabeled DNA probes can be directly prepared in remelted agarose using DNA gel slices cut from low melting point agarose gel. Using DNA gel slices cut from low melting point agarose gel, restriction fragments can be easily cloned into plasmids directly in remelted agarose. Ethidium bromide dye is used, so the gel has low background fluorescence.
2. Derivatives with low gel temperature have unique gel characteristics. The gel point is lower than 30 ℃, and the heavy melting point is higher than 65 ℃. Gel has excellent transparency and is suitable for preparing media containing thermal sensitive materials. Typical working concentration: 6-12g/l
3. It can perform excellent enzymatic reaction and clonal analysis in gel, and can also recover heat sensitive samples after electrophoretic reaction. Gel has good transparency, and is especially suitable for the preparation of culture media containing heat labile substances. It is recommended to prepare agarose microspheres.
【Product Specification】
Item | Specifications |
Appearance | white to off-white powder |
Solubility: | clear colorless solution at 2G plus 100ml water |
Water: | ≤10% |
Ash: | ≤0.5% |
Gel strength(1.5% Gel) : | ≥750 g/cm2 |
EEO(Electroendosmosis(-Mr) : | ≤0.1% |
Sulfate: | ≤0.1% |
Gelling point(1.5% Gel): | ≤33℃ |
Melting point(1.5% Gel): | ≤70℃ |
DNase: | NONE |
RNase: | NONE |
Protease: | NONE |
Endonuclease/ligase inhibitory factor: | NONE |